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Swine Artificial Insemination Procedures

As the swine industry scale and intensive level of continuous improvement, breeding sows has a direct impact on the level of the economic benefits of farmers. To raise the level of sow breeding the most direct and effective way is to use Artificial Insemination. Artificial insemination technology not only can greatly increase the conception rate of sows to accelerate the pace of improvement of pig breeds, and promote the improvement of pig production levels, but also significantly reduce the number of boars in the breeding and reduce costs. Artificial insemination of pigs in China in the early 50s of the 20th century a small number of institutions of higher learning, scientific research units to test the late 70s more widely used in the production, and achieved good effect. But our current artificial insemination rate of about 30% of the developed countries and 60% to 85% have a gap, therefore, also vigorously promoted. The procedure of artificial insemination have the following steps:

Prepared before semen collection semen room cleaning and disinfecting; regulation laboratory at room temperature to 25 . To set precise Cup, glass rod, thermometer, gauze, latex gloves and other equipment placed at 40 incubator preheated (except in summer); microscope regulate the focal length, keeping the temperature boxes microscopy 35 ~ 37 , containing glass film and into the microscope coverslip box preheat; prepared warm water around 25 .

Boar semen collection wrapper around the long hair cut, circumcised crowding, the inner product in urine, using a damp cloth dipped in potassium permanganate water back one way by the circumcision mouth clean. Semen release are generally simple to use the hand method. The donor members have to wear latex gloves, the boar semen collected in collection cup fine, fine cup set with 2 to 4 layer gauze hooded filtration at room temperature with low thermal insulation needs to set the cup, then quickly test room inspection, diluted and can be stored.

Clear liquid Quality Inspection Visual inspection semen normal gray or white color, the higher the concentration the more the color white; normal smell slightly fishy smell, or smell; generally weakly alkaline or neutral. Determination of sperm vitality

check is to ensure that sows impregnated an important technology. Specific methods of operation are: semen drops in the 38 constant temperature of the glass slide, a 10 point system to score, fresh semen survival rate greater than 0.6, storage of semen survival rate greater than 0.4. Morphological examination of sperm tails

, decapitation, a protoplast, the first large, double-headed, two-tailed, Orio, etc. are considered abnormal sperm. The rate of boar sperm fine not more than 18%, more than 20% of not suitable for Insemination.

Inspection is to determine the concentration of checking the concentration of sperm dilution of the important basis of visual observation through the microscope method, the semen can be divided into dense, medium and sparse three levels. In addition, the board can also be obtained by blood count, sperm density gauge accurate calculation of sperm density. The most commonly used artificial insemination sperm density of 0.5 million / ml, insemination of 60 to 80 ml, to ensure effective sperm 3000000000 ~ 4000000000.

Semen dilution, repackaging, and save the pig semen dilution formula There are many, according to their effects, preservation methods, and economical choice to decide which. The formula commonly used is: glucose 5 to 6 grams of sodium citrate 0.3 to 0.5 grams of EDTA or EDTA 0.1 g, increased to 100 ml with distilled water, the scale of pig farms in general by 1000 ml volume of preparation. OK well after dilution, the corresponding diluent into the semen along Beibi slow, slow rock edge chamfer; diluted semen in accordance with the specifications of separate packaging, the sperm will be kept at room temperature 1 to 2 hours slow release after 17 temperature into the refrigerator. Estrus in sows

Insemination fit accurately the quality of sperm transport to the cervix, the pregnancy rate is high to ensure an important link in sows. Insemination of specific methods of operation are: Remove the stored semen, Sperm viability inspection, after passing the slow warming to 37 for insemination. Insemination should sow vagina before a clean wipe with a towel, when the first upward into the vas deferens, in accordance with anticlockwise cervical arrival in sows.

In short, the proper use of artificial insemination technology can greatly enhance the sow conception rate, improve the quality of pigs to reduce the spread of disease, reduce production costs and the majority of farmers to achieve greater economic benefits, is worth promoting.

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